antibody microarray service Search Results


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liquid chromatography  (KCAS Bioanalytical and Biomarker Services)
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kcas bio analytical  (KCAS Bioanalytical and Biomarker Services)
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antibody microarray service  (Kinexus Bioinformatics Corporation)
90
Kinexus Bioinformatics Corporation antibody microarray service
Kinexus antibody <t>microarray.</t> Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.
Antibody Microarray Service, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody microarray service - by Bioz Stars, 2026-03
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expanded kinextm 800 antibody microarray service  (Kinexus Bioinformatics Corporation)
90
Kinexus Bioinformatics Corporation expanded kinextm 800 antibody microarray service
Kinexus antibody <t>microarray.</t> Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.
Expanded Kinextm 800 Antibody Microarray Service, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Kinexus antibody microarray. Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.

Journal: International Journal of Molecular Medicine

Article Title: Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion

doi: 10.3892/ijmm.2016.2601

Figure Lengend Snippet: Kinexus antibody microarray. Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.

Article Snippet: The extracts were analyzed by the Kinexus antibody microarray service, which utilizes >650 verified antibodies against most well-studied signaling proteins, including phospholipase Cγ, PI3-kinase, STAT and Akt.

Techniques: Microarray, Western Blot, Migration, Molecular Weight